ID 111015
タイトル別表記
マイクロアレイを用いた切除不能進行胃癌におけるS-1,シスプラチン,ドセタキセル3剤併用化学療法の治療効果予測因子についての検討
Response Predictors of DCS Therapy in Gastric Cancer
著者
Tanahashi, Toshihito Tokushima University
Aoyagi, Eriko Tokushima University
キーワード
Gastric cancer
Microarray
Chemotherapy
胃癌
化学療法
効果予測因子
マイクロアレイ
遺伝子
資料タイプ
学位論文
抄録
Objectives: The aim of this study was to identify biomarkers for predicting the efficacy of docetaxel, cisplatin, and S-1 (DCS) therapy for advanced gastric cancer using microarrays of biopsy specimens before chemotherapy. Methods: Nineteen samples were taken from 19 patients with unresectable metastatic gastric cancer who received DCS as a first-line therapy. Laser capture microdissection was performed, and total cellular RNA was extracted from each microdissected sample. Whole-gene expression was analyzed by microarray, and the difference in mRNA expression observed with the microarrays was confirmed by quantitative real-time PCR. Immunohistochemical staining was performed using clinical tissue sections obtained by endoscopic biopsy. Results: Eleven patients were identified as early responders and 8 patients as nonresponders to DCS therapy. Twenty-nine genes showed significant differences in relative expression ratios between tumor and normal tissues. A classifier set of 29 genes had high accuracy (94.7%) for distinguishing gene expression between 11 early responders and 8 nonresponders. Decreasing the size of the classifier set to 4 genes (PDGFB, PCGF3, CISH, and ANXA5) increased the accuracy to 100%. Expression levels by real-time PCR for validation were well correlated with those 4 genes in microarrays. Conclusion: The genes identified may serve as efficient biomarkers for personalized cancer-targeted therapy.
掲載誌名
Oncology
ISSN
00302414
14230232
cat書誌ID
AA00764146
AA12784865
出版者
Karger
93
2
開始ページ
127
終了ページ
135
発行日
2017-05-17
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shinji Kitamuraの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2017 S. Karger AG, Basel
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
乙第2097号
学位記番号
乙医第1751号
学位授与年月日
2017-11-30
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院