ID 83488
著者
ハヤシ, シゲヒト Second Department of Internal Medicine, The University of Tokushima School of Medicine
六車, 直樹 Second Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
バンドウ, テルミ Second Department of Internal Medicine, The University of Tokushima School of Medicine
タオカ, サトコ Second Department of Internal Medicine, The University of Tokushima School of Medicine
伊東, 進 Second Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
イイ, クニオ First Department of Pathology, The University of Tokushima School of Medicine
キーワード
micro-cancer
diagnostic method
indocyanine green (ICG) derivatives
vital immunohistochemical staining
資料タイプ
学術雑誌論文
抄録
It becomes possible to establish a novel diagnostic method for micro-cancer by modulating the signals from the lesion, if lesions can be labeled with substances which can be detected by video endoscopy. The authors have already succeeded in synthesizing indocyanine green (ICG) derivatives for a fluorescent labeling substance which emits near-infrared rays. Before the antibodies labeled by these substances can be used, it is necessary to establish a method of vital immunohistochemical staining. So, we inves-tigated the responses of antibodies exposed to non-fixed fresh tissue specimens as a basic study on vital immunohistochemical staining. The responses of fresh esophageal and gastric specimens (biopsied or surgically resected) to immunohistochemical staining with anti-epithelial membrane antigen (EMA) antibodies under various conditions using the ABC method were examined. These tissue specimens were stained immunohistochemically, and incubated with anti-EMA antibodies for 10 and 30 minutes (esophagus), and for 60 and 120 minutes (stomach) at 37°C. These results suggest that vital immunohistochemical staining is possible under optimum conditions. If an infrared fluorescent endoscopy catching this excited fluorescence can be developed, it will be possible to establish a new endoscopic diagnostic method on the basis of vital immunohistochemical staining.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
46
3-4
開始ページ
178
終了ページ
185
並び順
178
発行日
1999
備考
EDB ID
フルテキストファイル
言語
eng
部局
医学系