ID 110665
著者
矢野, 聖二 Third Department of Internal Medicine, The University of Tokushima School of Medicine
楊河, 宏章 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
埴淵, 昌毅 Third Department of Internal Medicine, The University of Tokushima School of Medicine KAKEN研究者をさがす
Pai, Kalpana Third Department of Internal Medicine, The University of Tokushima School of Medicine
西岡, 安彦 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ツルオ, タカシ Institute of Molecular and Cellular Biosciences, The University of Tokyo
曽根, 三郎 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
multidrug resistance
P-glycoprotein
ganglioside GM2
ADCC
cyclosporin A
資料タイプ
学術雑誌論文
抄録
A P-glycoprotein (P-gp) inhibitor, cyclosporin A (CsA) was found to enhance the susceptibility of multidrug resistant (MDR) cancer cells to anti-P-gp antibody-dependent cellular cytolysis (ADCC) by monocytes, but the exact mechanism is unknown. In this study, we examined whether CsA enhanced the susceptibility of MDR cells through its inhibitory effect of P-gp function by using anti-ganglioside GM2 (GM2) monoclonal antibody (Ab), KM966, instead of anti-P-gp Ab, MRK16. Monocyte-ADCC induced by both KM966 and MRK16 against P-gp positive human MDR ovarian cancer cells was significantly augmented by addition of CsA. KM966, but not MRK16, induced monocyte-ADCC against P-gp negative human ovarian cancer cells and CsA enhanced this ADCC activity, indicating that suppressive effect of P-gp function by CsA was not essential to the enhancement of ADCC. Moreover, pretreatment of tumor cells with CsA augmented their susceptibility to monocyte-ADCC irrespective of P-gp expression. Interestingly, KM966 or MRK16 induced monocyte-ADCC against various human lung cancer cells expressing either GM2 or P-gp, but CsA did not affect these ADCC. These findings suggest that CsA may enhance the susceptibility to the monocyte-ADCC of ovarian cancer cells, but not of lung cancer cells, irrespective of its suppressive effect of P-gp function.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
44
3-4
開始ページ
185
終了ページ
191
並び順
185
発行日
1998
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
病院
医学系