アクセス数 : ?
ダウンロード数 : ?
ID 114154
タイトル別表記
Epigallocatechin 3-gallateのNrf2経路を介したマウス膵島細胞保護作用に関する検討
protective effect of EGCG on mouse islets
著者
髙田, 厚史 徳島大学大学院医科学教育部(医学専攻)
和田, 佑馬 Tokushima University
キーワード
Epigallocatechin 3-gallate (EGCG)
Nrf2
islet transplantation
Reactive oxygen species (ROS)
Pre-conditioning
Nuclear factor erythroid 2-related factor 2 (Nrf2)
Reactive oxygen species (ROS) production
資料タイプ
学位論文
抄録
Purpose: Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation.
Methods: The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 μM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 μM and control group were transplanted under the kidney capsule of streptozotocin (STZ)-induced diabetic ICR mice.
Results: The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p<0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p<0.05), the ROS production was lower (p<0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p<0.01).
Conclusion: EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.
掲載誌名
Surgery Today
ISSN
09411291
14362813
cat書誌ID
AA10824685
出版者
Springer|Japan Surgical Society
49
6
開始ページ
536
終了ページ
545
発行日
2019-02-07
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Atsushi Takataの学位論文として提出され,学位審査・授与の対象となっている。
This is a post-peer-review, pre-copyedit version of an article published in Surgery Today. The final authenticated version is available online at: https://doi.org/10.1007/s00595-019-1761-0
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3342号
学位記番号
甲医第1434号
学位授与年月日
2019-12-26
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系