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ID 115574
タイトル別表記
EFFECT OF FERULIC ACID ON PIG OOCYTES
著者
Nhien, Nguyen Thi Tokushima University
Hirano, Takayuki Tokushima University
Kunihara, Toshiki Tokushima University
キーワード
antioxidant
developmental competence
embryo
oxidative stress
trans-ferulic acid
資料タイプ
学術雑誌論文
抄録
The value of laboratory and genetically-modified pigs is becoming increasingly clear; however, their in vitro development remains inefficient. Trans-ferulic acid (trans-FA) is an aromatic compound that is abundant in plant cell walls, and which exhibits antioxidant effects in vitro. Trans-FA is known to improve sperm viability and motility; however, its effects on porcine oocytes are unknown. Our aim was to investigate the effects of trans-FA supplementation during in vitro maturation on the meiotic and developmental competence of porcine oocytes. Oocytes were matured either without (control) or with trans-FA (10, 100 and 1,000 µM), fertilized, and cultured in vitro for 7 days. The maturation rate of oocytes cultured with 10 µM trans-FA (81.6%) was significantly higher than that of controls (65.0%; P<0.05). The fertilization rate of oocytes matured with 10 µM trans-FA (57.4%) was also significantly higher than that of controls (32.7%) and oocytes cultured with other concentrations (33.1% and 22.7% for 100 and 1,000 µM, respectively; P<0.05). Moreover, the blastocyst formation rate of oocytes matured with 10 µM trans-FA (6.9%) was significantly higher than that of controls (2.3%; P<0.05). Our results suggest that in vitro maturation with 10 µM trans-FA is beneficial for the in vitro production of porcine embryos and has the potential to improve production system.
掲載誌名
Journal of Veterinary Medical Science
ISSN
13477439
出版者
The Japanese Society of Veterinary Science
80
6
開始ページ
1007
終了ページ
1011
発行日
2018-05-16
権利情報
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
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フルテキストファイル
言語
eng
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出版社版
部局
生物資源系