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ID 117826
タイトル別表記
Analysis of hydroxy sphingomyelin
著者
Ali, Hanif Tokushima University
Yamashita, Ryouhei Tokushima University
Morishige, Jun-ichi Kanazawa University
Morito, Katsuya Tokushima University
Kakiuchi, Naoya Tokushima University
キーワード
Sphingomyelin
Molecular species
liquid chromatography-tandem mass spectrometry
α-Hydroxy fatty acid
Skin
資料タイプ
学術雑誌論文
抄録
Sphingomyelin (SM) with N-α-hydroxy fatty acyl residues (hSM) has been shown to occur in mammalian skin and digestive epithelia. However, the metabolism and physiological relevance of this characteristic SM species have not been fully elucidated yet. Here, we show methods for mass spectrometric characterization and quantification of hSM. The hSM in mouse skin was isolated by TLC. The hydroxy hexadecanoyl residue was confirmed by electron impact ionization-induced fragmentation in gas chromatography-mass spectrometry. Mass shift analysis of acetylated hSM by time of flight mass spectrometry revealed the number of hydroxyl groups in the molecule. After correcting the difference in detection efficacy, hSM in mouse skin and intestinal mucosa were quantified by liquid chromatography-tandem mass spectrometry, and found to be 16.5 ± 2.0 and 0.8 ± 0.4 nmol/μmol phospholipid, respectively. The methods described here are applicable to biological experiments on hSM in epithelia of the body surface and digestive tract.
掲載誌名
Lipids
ISSN
15589307
cat書誌ID
AA00275467
出版者
American Oil Chemists' Society|Wiley
56
2
開始ページ
181
終了ページ
188
発行日
2020-09-30
権利情報
This is the peer reviewed version of the following article: Ali, H., Yamashita, R., Morishige, J.-i., Morito, K., Kakiuchi, N., Hayashi, J., Aihara, M., Kawakami, R., Tsuchiya, K. and Tanaka, T. (2021), Mass Spectrometric Analysis of Sphingomyelin with N-α-Hydroxy Fatty Acyl Residue in Mouse Tissues. Lipids, 56: 181-188., which has been published in final form at https://doi.org/10.1002/lipd.12285. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系
薬学系