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ID 110114
タイトル別表記
胎生期に低栄養を経験した雌ラットにおける加齢時の食欲促進因子および抑制因子の発現に関する研究
著者
ツンガラグスブド, アルタンフウ 徳島大学大学院医科学教育部(医学専攻)
Munkhzaya, Munkhsaikhan Tokushima University
Mayila, Yiliyasi Tokushima University
Kawami, Takako Tokushima University
キーワード
FGR
Orexigenic factor
Anorexigenic factor
DOHaD
NPY
資料タイプ
学位論文
抄録
Fetal growth retardation, which affects short- and long-term fetal brain development, is associated with metabolic, hematological, and thermal disturbances, which can increase the risk of metabolic syndrome later in life. Orexigenic and anorexigenic factors regulate food intake and energy expenditure. We studied how the expression of these factors was affected by food deprivation (FD) in middle-aged female rats that had been subjected to prenatal undernutrition. Eight pregnant rats were divided into two groups,the normal nutrition (NN) (n = 4) group and the undernutrition (UN) (n = 4) group, which received 50%(approximately 11 g) of the daily food intake of the normal nutrition rats from day 13 of pregnancy to delivery. The pups from these dams were defined as the maternal NN (mNN) and maternal UN (mUN) groups, respectively. After weaning, all of the pups were housed and allowed ad libitum access to food and water. At the age of 6 months, both groups of pups were sub-divided into three groups. One group was allowed to consume normal amounts of food (Fed), and the other two groups were subjected to 24 h or 48 h FD (n = 7–8 per group). The rats’ serum leptin levels and hypothalamic mRNA expression levels of various orexigenic or anorexigenic factors were measured. In both the mNN and mUN rats, the serum leptin levels of the 24 h and 48 h FD groups tended to be lower than those of the Fed group, and the serum leptin levels of the 24 h FD mUN rats and the Fed mUN rats differed significantly. The hypothalamic neuropeptide Y (NPY) mRNA expression levels of the 24 h and 48 h FD groups were significantly higher in the mUN rats than in the mNN rats. In addition, among the mUN rats the hypothalamic NPY mRNA expression levels of the 48 h FD group were significantly higher than those of the Fed group. In both the mNN and mUN rats, prepro-orexin mRNA expression was lower in the 48 h FD group than in the corresponding Fed group. Among the mUN rats, the 48 h FD group exhibited significantly lower hypothalamic proopiomelanocortin (POMC) mRNA expression than the Fed group, and a similar tendency was seen among the mNN rats. Among the mNN rats, the 24 h FD group displayed significantly higher hypothalamic leptin receptor (OBRb) mRNA levels than the Fed group. However, no such differences were seen among the mUN rats. As a result, the hypothalamic OBRb mRNA expression levels of the mUN rats in the 24 h and 48 h FD groups were lower than those of the corresponding mNN rat groups. These findings indicate that rats that are subjected to prenatal undernutrition exhibit upregulated expression of orexigenic factors and are more sensitive to FD in middle age, which might increase their risk of developing metabolic disorders in later life.
掲載誌名
International Journal of Developmental Neuroscience
ISSN
07365748
cat書誌ID
AA10637655
AA11531026
出版者
Elsevier|International Society for Developmental Neuroscience
49
開始ページ
1
終了ページ
5
発行日
2015-12-15
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201705191009.pdf
論文本文:k3022_fulltext.pdf
本論文は, 著者Altankhuu Tungalagsuvdの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3022号
学位記番号
甲医第1326号
学位授与年月日
2017-03-23
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院