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ID 116080
タイトル別表記
SENSITIVITY OF MEIOTIC STAGE TO HYPERTHERMIA IN PIG OOCYTE
SENSITIVITY OF THE MEIOTIC STAGE OF PORCINE OOCYTES TO HYPERTHERMIA
著者
Nishio, Katsutoshi Tokushima University
Yamazaki, Mado Yamaguchi University
Taniguchi, Masayasu Yamaguchi University
Besshi, Kazuhiko Taiho Pharmaceutical
Morita, Fumio Taiho Pharmaceutical
Kunihara, Toshiki Tokushima University
キーワード
Heat stress
maturation
meiotic stage
quality
sensitivity
資料タイプ
学術雑誌論文
抄録
The present study was conducted to clarify the meiotic stage of porcine oocytes having the highest sensitivity to hyperthermia during in vitro maturation by evaluating the meiotic competence and DNA damage. Oocytes were exposed to 41 °C for 12 h at various intervals during 48 h of maturation culture. When the oocytes were exposed to 41 °C from 12 to 24 h of the maturation culture, the proportion of oocytes reaching metaphase II (MII) decreased as compared to the control oocytes cultured at 38.5 °C (P < 0.05). Moreover, the proportions of DNA fragmentation in all oocytes exposed to 41 °C in each culture period after 12 h from the start of maturation culture were significantly higher (P < 0.05) than for the control oocytes. When the meiotic stage of oocytes cultured at 38.5 °C between 12 and 24 h was examined, the majority of oocytes remained at the germinal vesicle (GV) stage at 12 h and approximately half of the oocytes reached metaphase I (MI) at 24 h. These results indicate that the meiotic stage of porcine oocytes having the highest sensitivity to hyperthermia during in vitro maturation is a transition period from the GV stage to the MI stage.
掲載誌名
Acta Veterinaria Hungarica
ISSN
02366290
15882705
cat書誌ID
AA10499223
出版者
Akadémiai Kiadó
65
1
開始ページ
115
終了ページ
123
発行日
2017-03-01
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系
先端酵素学研究所