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ID 106432
著者
サイトウ, ショウヘイ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
フクナガ, エリ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
ホンダ, サリ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
金丸, 芳 Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
佐藤, 征弥 Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
小山, 保夫 Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima KAKEN研究者をさがす
キーワード
nonivamide
intracellular Ca2+
lymphocyte
capsaicin
資料タイプ
紀要論文
抄録
Effect of nonivamide, a natural analog of capsaicin, on intracellular Ca2+ level of rat thymocytes was examined using a flow-cytometric technique with appropriate fluorescent probes in order to further characterize the cytotoxicity because nonivamide can be used as an active intergradient of antifouling paints. Nonivamide at concentrations ranging from 30 μM to 300 μM significantly increased the intensity of Fluo-3 fluorescence. The potency of 100 μM nonivamide to increase the fluorescence was similar to that of 100 μM capsaicin. The increase in Fluo-3 fluorescence by 100 μM nonivamide was attenuated under an external Ca2+-free condition. Nonivamide at 100 μM also increased the intensity of Fluo-3 fluorescence in the continued presence of 100 μM capsaicin. It is suggested that nonivamide at high micromolar concentrations increases intracellular Ca2+ level via the activation of vanilloid receptors. Nonivamide concentrations (30 μM or more) that increase intracellular Ca2+ level in rat thymocytes are comparable to those in algal cells. However, it is something hard to argue the implications in environmental science because nonivamide doesn’t seem to be released into environment in such a high concentration, and because bioaccumulation of nonivamide has not been reported.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
28
3
開始ページ
15
終了ページ
19
並び順
15
発行日
2014-07
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系