アクセス数 : ?
ダウンロード数 : ?
ID 106008
著者
クワハラ, ショウジ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
アラナミ, フミト Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
瀬川, 博子 Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
オニツカ, アケミ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
ホンダ, ナオコ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
トミナガ, リエコ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
ハナブサ, エツヨ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
カネコ, イチロウ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ヤマナカ, セツコ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
ササキ, ショウヘイ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
オオイ, アキコ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
ノムラ, ケンゴ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
タツミ, サワコ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
キド, シンスケ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
イトウ, ミキコ Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School|University of Hyogo School of Human Science and Environment
宮本, 賢一 Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
phosphate transporter
HHRH
Npt2c
資料タイプ
学術雑誌論文
抄録
Mutations in the SLC34A3 gene, a sodium-dependent inorganic phosphate (Pi) cotransporter, also referred to as NaPi IIc, causes hereditary hypophosphatemic rickets with hypercalciuria (HHRH), an autosomal recessive disorder. In human and rodent, NaPi IIc is mainly localized in the apical membrane of renal proximal tubular cells. In this study, we identified mouse NaPi IIc variant (Npt2c-v1) that lacks the part of the exon 3 sequence that includes the assumed translation initiation site of Npt2c. Microinjection of mouse Npt2c-v1 cRNA into Xenopus oocytes demonstrated that Npt2c-v1 showed sodium-dependent Pi cotransport activity. The characterization of pH dependency showed activation at extracellular alkaline-pH. Furthermore, Npt2c-v1 mediated Pi transport activity was significantly higher at any pH value than those of Npt2c. In an in vitro study, the localization of the Npt2c-v1 protein was detected in the apical membrane in opossum kidney cells. The expression of Npt2c-v1 mRNA was detected in the heart, spleen, testis, uterus, placenta, femur, cerebellum, hippocampus, diencephalon and brain stem of mouse. Using mouse bone primary cultured cells, we showed the expression of Npt2c-v1 mRNA. In addition, the Npt2c protein was detected in the spermatozoa head. Thus, Npt2c-v1 was expressed in extra-renal tissues such as epididymal spermatozoa and may function as a sodium-dependent phosphate transporter.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
59
1-2
開始ページ
116
終了ページ
126
並び順
116
発行日
2012-02
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系