ツジ, マサヒト Department of Urology, The University of Tokushima School of Medicine
カンダ, カズヤ Department of Urology, The University of Tokushima School of Medicine
ムラカミ, ヨシヒデ Department of Urology, The University of Tokushima School of Medicine
クロカワ, ヤスシ Department of Urology, The University of Tokushima School of Medicine
金山, 博臣 Department of Urology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
佐野, 壽昭 First Department of Pathology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
prostatic intraepithelial neoplasia
fluorescence in situ hybridization
Objective:We evaluated the biological properties of High-grade prostatic intraepithelial neoplasia (PIN) by immunohistochemistry and fluorescence in situ hybridization (FISH) analysis in relation to normal tissue and carcinoma lesions.
Materials and Methods:Immunohistochemical staining and FISH were performed on23formalin-fixed radical prostatectomy specimens taken from patients with PIN. Assays were performed using MIB-1, chromogranin A (CGA) and an anti-androgen receptor antibody (AR). A centromere probe for chromosome8was used to test for aneuploidy.
Results:The MIB-1index of cancerous specimens (16.2±10.5%) was significantly higher than that of benign (1.9±1.6%, p<0.0001) or PIN (4.0±4.5%, p<0.0001) specimens. The percentage of CGA positive cells was significantly lower in normal tissue (1.2±1.8%) than in PIN (3.5±2.9%, p=0.012) or carcinoma (5.4±4.9%, p=0.005) lesions. Positive staining for AR was consistently observed in the nuclei of both benign and malignant epithelial cells, but positive cytoplasmic staining was also seen in PIN epithelial cells. No significant difference in FISH detected anomalies were found between PIN and carcinoma specimens.
Conclusions:Our studies concerning proliferative activity, NE differentiation and chromosomal anomalies of prostatic specimens support the hypothesis that PIN is a biologically intermediate stage in the pathogenesis of prostatic carcinoma. The cellular distribution of AR was altered in PIN cells, but the role of AR in PIN is not yet clear.
The journal of medical investigation : JMI
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