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タイトル別表記
細胞診における探針エレクトロスプレーイオン化質量分析の有用性 : 肺癌培養細胞での検討
PESI-MS for Diagnostic Cytology
著者
森本, 友樹 徳島大学大学院医科学教育部(医学専攻)
Sumida, Satoshi Tokushima University
Kakimoto, Takumi Tokushima University
Cheng, Chunmei Novo Nordisk Research Centre China
キーワード
Mass spectrometry
Rapid cytological diagnosis
Probe electrospray ionization mass spectrometry
Lung cytopathology
資料タイプ
学位論文
抄録
Objectives: Cytology and histology are 2 indispensable diagnostic tools for cancer diagnosis, which are rapidly increasing in importance with aging populations. We applied mass spectrometry (MS) as a rapid approach for swiftly acquiring nonmorphological information of interested cells. Conventional MS, which primarily rely on promoting ionization by pre-applying a matrix to cells, has the drawback of time-consuming both on data acquisition and analysis. As an emerging method, probe electrospray ionization-MS (PESI-MS) with a dedicated probe is capable to pierce sample and measure specimen in small amounts, either liquid or solid, without the requirement for sample pretreatment. Furthermore, PESI-MS is timesaving compared to the conventional MS. Herein, we investigated the capability of PESI-MS to characterize the cell types derived from the respiratory tract of human tissues. Study Design: PESI-MS analyses with DPiMS-2020 were performed on various type of cultured cells including 5 lung squamous cell carcinomas, 5 lung adenocarcinomas, 5 small-cell carcinomas, 4 malignant mesotheliomas, and 2 normal controls. Results: Several characteristic peaks were detected at around m/z 200 and 800 that were common in all samples. As expected, partial least squares-discriminant analysis of PESI-MS data distinguished the cancer cell types from normal control cells. Moreover, distinct clusters divided squamous cell carcinoma from adenocarcinoma. Conclusion: PESI-MS presented a promising potential as a novel diagnostic modality for swiftly acquiring specific cytological information.
掲載誌名
Acta Cytologica
ISSN
00015547
19382650
cat書誌ID
AA00044384
AA12780809
出版者
Karger
65
5
開始ページ
430
終了ページ
439
発行日
2021-06-07
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Yuki Morimotoの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2021 S. Karger AG, Basel
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3535号
学位記番号
甲医第1502号
学位授与年月日
2021-09-09
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系