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ID 117543
タイトル別表記
結腸上皮細胞のin vitro基底膜形成における近傍線維芽細胞由来IV型コラーゲンの寄与
BM collagen IV deposition by proximal fibroblasts
著者
天宅, あや 徳島大学大学院医科栄養学研究科(医科栄養学専攻)
Sejima, Kurumi Tokushima University
Nagao, Toshiki Tokushima University
キーワード
Basement membrane
collagen IV
fibroblast
colon epithelium
co-culture
資料タイプ
学位論文
抄録
The basement membrane (BM) underlying epithelial tissue is a thin layer of extracellular matrix that governs tissue integrity and function. Epithelial BMs are generally assembled using BM components secreted from two origins: epithelium and stroma. Although de novo BM formation involves self-assembly processes of large proteins, it remains unclear how stroma-derived macromolecules are transported and assembled, specifically in the BM region. In this study, we established an in vitro co-culture model of BM formation in which DLD-1 human colon epithelial cells were cultured on top of collagen I gel containing human embryonic OUMS-36T-2 fibroblasts as stromal cells. A distinct feature of our system is represented by OUMS-36T-2 cells which are almost exclusively responsible for synthesis of collagen IV, a major BM component. Exploiting this advantage, we found that collagen IV incorporation was significantly impaired in culture conditions where OUMS-36T-2 cells were not allowed to directly contact DLD-1 cells. Soluble collagen IV, once diluted in the culture medium, did not accumulate in the BM region efficiently. Live imaging of fluorescently tagged collagen IV revealed that OUMS-36T-2 cells deposited collagen IV aggregates directly onto the basal surface of DLD-1 cells. Collectively, these results indicate a novel mode of collagen IV deposition in which fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly during BM formation.
掲載誌名
The FEBS Journal
ISSN
17424658
1742464X
cat書誌ID
AA11998513
AA12092067
出版者
Federation of European Biochemical Societies|Wiley
289
23
開始ページ
7466
終了ページ
7485
発行日
2022-06-22
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Aya Tentakuの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This is the peer reviewed version of the following article: Tentaku, A., Kurisu, S., Sejima, K., Nagao, T., Takahashi, A. and Yonemura, S. (2022), Proximal deposition of collagen IV by fibroblasts contributes to basement membrane formation by colon epithelial cells in vitro. FEBS J., which has been published in final form at https://doi.org/10.1111/febs.16559. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3657号
学位記番号
甲栄第293号
学位授与年月日
2022-09-22
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系