直近一年間の累計
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ID 111267
著者
Enomoto, Kei The University of Tokyo
Watanabe-Susaki, Kanako Advanced Industrial Science and Technology (AIST)
Kowno, Megumi The University of Tsukuba
Takada, Hitomi Advanced Industrial Science and Technology (AIST)
印東, 厚 The University of Tokyo
Yamanaka, Yuko Yokohama City University
Hirano, Hisashi Yokohama City University
杉野, 弘 Advanced Industrial Science and Technology (AIST)
Asashima, Makoto Advanced Industrial Science and Technology (AIST)|The University of Tokyo|Japan Science and Technology Agency (JST)
栗崎, 晃 Advanced Industrial Science and Technology (AIST)|The University of Tsukuba
キーワード
Proteomics
ES cells
pluripotency
differentiation
proteins
資料タイプ
学術雑誌論文
抄録
Mammalian pluripotent stem cells possess properties of self-renewal and pluripotency. These abilities are maintained by the strict regulation of pluripotent stem cell-specific transcription factor network and unique properties of chromatin in the stem cells. Although these major signaling pathways robustly control the characteristics of stem cells, other regulatory factors, such as metabolic pathways, are also known to modulate stem cell proliferation and differentiation. In this study, we fractionated protein samples from mouse embryonic stem (ES) cells cultured with or without the leukemia inhibitory factor (LIF). Protein expression was quantified by 2-dimensional differential gel electrophoresis (2D-DIGE). In total, 44 proteins were identified as being differentially expressed in the pluripotent stem cells and the differentiated cells. Surprisingly, half of the identified proteins were the proteins localized in mitochondria, which supply cellular energy and regulate cell cycle, development, and cell death. Some of these identified proteins are involved in the metabolic function and the regulation of pluripotency. Further analysis of the identified proteins could provide new information for the manipulation of pluripotency in ES cells.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
62
3-4
開始ページ
130
終了ページ
136
並び順
130
発行日
2015-08
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所