The Effectiveness of Human Prathyroid Hormone and Low-intensity Pulsed Ultrasound on the Fracture Healing in Osteoporotic Bones

Osteoporotic fracture has become a major public health problem, and until today, the treatments available are not satisfactory. While there is growing evidence to support the individual treatment of parathyroid hormone (PTH) administration and low-intensity pulsed ultrasound (LIPUS) exposure as respectively systemic and local therapies during osteoporotic fracture healing, their effects have not yet been investigated when introduced concurrently. This study aimed to evaluate the effects of combined treatment with PTH (1–34) and LIPUS on fracture healing in ovariectomized (OVX) rats. Thirty-two, 12-week-old female Sprague–Dawley rats were OVX to induce osteoporosis. After 12 weeks, the rats underwent surgery to create bilateral mid-diaphyseal fractures of proximal tibiae. All animals were randomly divided into 4 groups (n = 8 for each): control group as placebo, PTH group, LIPUS group, and combined group. PTH group had PTH administration at a dose of 30 lg/kg/day for 3 days/week for 6 weeks. LIPUS group received ultrasound 5 days/week for 20 min/day for 6 weeks and combined group had both PTH administration and LIPUS exposure for 6 weeks. Fracture healing was observed weekly by anteroposterior radiography and micro-CT. Five weeks after the fracture, the tibia were harvested to permit histological assessments and at week 6, for mechanical property of the fracture callus. Micro-CT showed that the PTH and combined groups exhibited significantly higher BMD and trabecular bone integrity than control group at weeks 4–6. Radiography, fracture healing score and mean callus area indicated that the combined group revealed better healing processes than the individual groups. Mechanically, bending moment to failure was significantly higher in LIPUS, PTH and combined groups than in control group. These data suggest that the combined treatment of PTH and LIPUS have been shown to accelerate fracture bone healing and enhance bone properties rather than single agent therapy, and may be considered as a treatment remedy for fracture healing in postmenopausal osteoporosis.

内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201611141025.pdf
審査要旨 : LID201611141026.pdf
論文本文 : k2976_fulltext.pdf
著者の申請により要約(2016-11-14公開)に替えて論文全文を公開(2019-06-05)
本論文は, 著者KARIMA Q. MANSJURの学位論文として提出され, 学位審査・授与の対象となっている。

© Biomedical Engineering Society 2016
This is a post-peer-review, pre-copyedit version of an article published in Annals of Biomedical Engineering.
The final authenticated version is available online at https://doi.org/10.1007/s10439-015-1533-y