ID 101807
Title Transcription
ナトリウム イゾンセイ リンサン トランスポーター IIa ガタ NaPi-IIa ノ フクコウジョウセン ホルモン PHT ニヨル チョウセツ キコウ
Title Alternative
Translocational regulation of type IIa sodium-dependent phosphate transporter by parathyroid hormone.
Author
Nashiki, Kunitaka Department of Clinical Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School
Takeichi, Tomoko Department of Clinical Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School
Taketani, Yutaka Department of Clinical Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takeda, Eiji Department of Clinical Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
caveolae-like membrane domains
NaPi-IIa
phosphate transporter
PTH
PKA
PKC
Content Type
Journal Article
Description
Parathyroid hormone (PTH) is the most potent and important regulator of the type II a sodium-dependent phosphate transporter (NaPi-IIa), which plays a key role in renal phosphate reabsorption and maintaining inorganic phosphate homeostasis. PTH inhibits the NaPi-IIa activity by stimulation of translocation from the apical plasma membrane to the intracellular organelle. In this paper, we investigated the mechanism of the translo-cation of NaPi-IIa from the apical plasma membrane by cell fractionation analysis using OK-N2 cells that stably express human NaPi-IIa established from opossum kidney cells (OK-P cells). NaPi-IIa was mostly localized in the caveolae-like membrane domains of the plasma membrane in OK-N2 cells We also clarified that PTH activated both PKA and PKC, and these kinases markedly increased the phosphorylation of their 80kDa and 250kDa substrates on the caveolae-like membrane domains ; we identified ezrin as a candidate protein for the 80kDa substrate. We conclude that caveolae-like membrane domains play an important role in the targeting, translocation, and signal compartmentalization for the translocational regulation of NaPi-IIa in renal proximal tubular cells.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
60
Issue
5-6
Start Page
185
End Page
186
Sort Key
185
Published Date
2004-12-20
Remark
FullText File
language
jpn
departments
Medical Sciences