ID 106008
Author
Kuwahara, Shoji Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Aranami, Fumito Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Segawa, Hiroko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Onitsuka, Akemi Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Honda, Naoko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Tominaga, Rieko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Hanabusa, Etsuyo Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Kaneko, Ichiro Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yamanaka, Setsuko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Sasaki, Shohei Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Ohi, Akiko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Nomura, Kengo Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Tatsumi, Sawako Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Kido, Shinsuke Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Ito, Mikiko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School|University of Hyogo School of Human Science and Environment
Miyamoto, Ken-ichi Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
phosphate transporter
HHRH
Npt2c
Content Type
Journal Article
Description
Mutations in the SLC34A3 gene, a sodium-dependent inorganic phosphate (Pi) cotransporter, also referred to as NaPi IIc, causes hereditary hypophosphatemic rickets with hypercalciuria (HHRH), an autosomal recessive disorder. In human and rodent, NaPi IIc is mainly localized in the apical membrane of renal proximal tubular cells. In this study, we identified mouse NaPi IIc variant (Npt2c-v1) that lacks the part of the exon 3 sequence that includes the assumed translation initiation site of Npt2c. Microinjection of mouse Npt2c-v1 cRNA into Xenopus oocytes demonstrated that Npt2c-v1 showed sodium-dependent Pi cotransport activity. The characterization of pH dependency showed activation at extracellular alkaline-pH. Furthermore, Npt2c-v1 mediated Pi transport activity was significantly higher at any pH value than those of Npt2c. In an in vitro study, the localization of the Npt2c-v1 protein was detected in the apical membrane in opossum kidney cells. The expression of Npt2c-v1 mRNA was detected in the heart, spleen, testis, uterus, placenta, femur, cerebellum, hippocampus, diencephalon and brain stem of mouse. Using mouse bone primary cultured cells, we showed the expression of Npt2c-v1 mRNA. In addition, the Npt2c protein was detected in the spermatozoa head. Thus, Npt2c-v1 was expressed in extra-renal tissues such as epididymal spermatozoa and may function as a sodium-dependent phosphate transporter.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
59
Issue
1-2
Start Page
116
End Page
126
Sort Key
116
Published Date
2012-02
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences