ID 106016
Author
Aki, Kensaku Subdivision of Biomedical Laboratory Sciences, Graduate School of Health Sciences, the University of Tokushima|Department of Cells and Immunity Analytics, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Izumi, Azusa Subdivision of Biomedical Laboratory Sciences, Graduate School of Health Sciences, the University of Tokushima|Department of Cells and Immunity Analytics, Institute of Health Biosciences, the University of Tokushima Graduate School
Hosoi, Eiji Department of Cells and Immunity Analytics, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
ABO blood group
flow cytometric analysis
cisAB
DNA typing
PASA : PCR amplification of specific alleles
Content Type
Journal Article
Description
ABO antigens are oligosaccharide antigens, and are widely distributed on red blood and tissue cells as well as in saliva and body fluid. Therefore, these antigens are important not only for blood transfusion, but also for tissue cell and organ transplantations. Also, blood, hair, and seminal fluid are important sources of evidence at crime scenes, and these antigens are some of the most important markers for personal identification in forensic investigations. Here, we describe the development and use of quantitative analysis of A, B, and H antigens on red blood cells by employing flow cytometric analysis and the ABO genotyping method based on PCR-amplification of specific alleles (PASA) within DNA, especially from blood and saliva. In this study, flow cytometric analysis could be used to compare the differences between the expression of A and/or B and H antigens on red blood cells with various phenotypes, and the PASA method was able to determine the genotype of the type cisA2B3 pedigree using only DNA extracted from saliva. These analysis methods are simple and useful for judging the ABO blood group system and genotyping, and are used widely throughout research and clinical laboratories and forensic fields.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
59
Issue
1-2
Start Page
143
End Page
151
Sort Key
143
Published Date
2012-02
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences