ID 106314
Title Alternative
成長軟骨板において膜フィルターで血管侵入を阻害すると肥大軟骨細胞は増殖し骨芽細胞様細胞に分化する。
Author
Enishi, Tetsuya Department of Orthopedics, Institute of Health Biosciences, Tokushima University Graduate School KAKEN Search Researchers
Content Type
Thesis or Dissertation
Description
The fate of hypertrophic chondrocytes during endochondral ossification remains controversial. It has long been thought that the calcified cartilage is invaded by blood vessels and that new bone is deposited on the surface of the eroded cartilage by newly arrived cells. The present study was designed to determine whether hypertrophic chondrocytes were destined to die or could survive to participate in new bone formation. In a rabbit experiment, a membrane filter with a pore size of 1 mm was inserted in the middle of the hypertrophic zone of the distal growth plate of ulna. In 33 of 37 animals, vascular invasion was successfully interposed by the membrane filter. During 8 days, the cartilage growth plate was enlarged, making the thickness 3-fold greater than that of the nonoperated control side. Histological examination demonstrated that the hypertrophic zone was exclusively elongated. At the terminal end of the growth plate, hypertrophic chondrocytes extruded from their territorial matrix into the open cavity on the surface of the membrane filter. The progenies of hypertrophic chondrocytes (PHCs) were PCNA positive and caspase-3 negative. In situ hybridization studies demonstrated that PHCs did not express cartilage matrix proteins anymore but expressed bone matrix proteins. Immunohistochemical studies also emonstrated that the new matrix produced by PHCs contained type I collagen, osteonectin, and osteocalcin. Based on these results, we concluded that hypertrophic chondrocytes switched into bone-forming cells after vascular invasion was interposed in the normal growth plate.
Published Date
2014-10-23
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201411041001.pdf
論文本文 : LID201411041002.pdf
Copyright: © 2014 Enishi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Published Source
PloS one (2014) Vol.9 Issue.8 e104638 (DOI: 10.1371/journal.pone.0104638)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2778号
Diploma Number
甲医第1223号
Granted Date
2014-10-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital