ID 106363
Author
Higashimoto, Minoru Faculty of Pharmaceutical Sciences, Tokushima Bunri University|AWA Laboratory of Health-Food Drinks
Isoyama, Naohiro Faculty of Pharmaceutical Sciences, Tokushima Bunri University
Ishibashi, Satoshi Faculty of Pharmaceutical Sciences, Tokushima Bunri University
Ogawa, Naoko Faculty of Human Life Sciences, Tokushima Bunri University
Takiguchi, Masufumi Faculty of Pharmaceutical Sciences, Tokushima Bunri University|Faculty of Pharmaceutical Sciences, Hiroshima International University
Suzuki, Shinya Faculty of Pharmaceutical Sciences, Tokushima Bunri University
Ohnishi, Yoshinari Graduate School of Medicine, the University of Tokushima Tokushima University Educator and Researcher Directory
Sato, Masao Faculty of Pharmaceutical Sciences, Tokushima Bunri University
Keywords
metallothionein
repeated mild stress
DNA damage
dyslipidemia
Content Type
Journal Article
Description
The effects of repeated mild stress on DNA and lipid metabolic damages in multiple organs of dyslipidemic mice, and the preventive role of metallothionein (MT) were investigated. Female adult wild-type and MT-null mice fed high-fat diet (HFD) or standard diet (STD) were repeatedly subjected to fasting or restraint for three weeks. The liver, pancreas, spleen, bone marrow and serum samples were taken for evaluating DNA damage, MT, glutathione (GSH), corticosterone, carnitine and adiponectin. Body weights of restraint groups were reduced with the intensity of stress increased, even if the energy intakes were higher than those of STD group. Hepatic GSH levels were reduced in HFD control group and were further reduced in stress groups, especially in restraint groups, while the hepatic MT and serum corticosterone levels were increased in concert with the intensity of stress. Cellular DNA damages were generally increased by the restraint stress, especially in MT-null mice. Hepatic carnitine levels of MT-null mice were markedly lower than those of wild-type mice. The data suggest that MT plays a preventive role by acting as an antioxidant in corporation with GSH decreased by repeated stress and that MT may be an essential factor for inducing carnitine under the stress.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
60
Issue
3-4
Start Page
240
End Page
248
Sort Key
240
Published Date
2013-08
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences