ID 106365
Author
Kashiwagi, Masayuki Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Ishigami, Akiko Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima, Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hara, Kenji Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Matsusue, Aya Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Waters, Brian Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Takayama, Mio Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Tokunaga, Itsuo Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima, Graduate School
Nishimura, Akiyoshi Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima, Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kubo, Shin-ichi Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Keywords
coma blister
sweat gland degeneration
apoptosis
intoxication
forensic autopsy cases
Content Type
Journal Article
Description
The erythematous patches and vesicles that are observed in coma patients, usually from an overdose of medication, are known as coma blisters. However, it is unknown whether the degenerated sweat gland is a necrosis or apoptosis. We immunohistochemically examined such skin lesions to investigate the characteristics and pathogenesis of the coma blister. Skin lesions were obtained from a forensic autopsy case, a woman in her thirties, of caffeine intoxication. Those lesions were observed in the left femoral, the lower left thigh, and the right knee. Histologically, the skin lesions showed that the keratinocytes had necrosed and the epidermis was thin in some areas. Eccrine sweat gland degeneration was observed. Obvious inflammatory cell infiltrations were not detected. Immunohistochemically, we stained each skin lesion against CD3, CD8, CD45RO, cytokeratin, 70 kD heat shock protein, ubiquitin, 150 kD oxygen regulated protein, and caspase-cleaved keratin 18 neo-epitope M30. They were also stained with an in situ apoptosis detection kit. Degenerated sweat glands featured CD45RO and M30 immunoreactivity. Immunohistochemical staining for CD45RO, CK-L, and M30 might be useful to observe sweat gland degeneration in the coma blister. Therefore, the apoptosis might be related to coma blisters and sweat gland degenerations.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
60
Issue
3-4
Start Page
256
End Page
261
Sort Key
256
Published Date
2013-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences