ID 106432
Author
Saitoh, Shohei Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
Fukunaga, Eri Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
Honda, Sari Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
Kanemaru, Kaori Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Satoh, Masaya Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Oyama, Yasuo Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
nonivamide
intracellular Ca2+
lymphocyte
capsaicin
Content Type
Departmental Bulletin Paper
Description
Effect of nonivamide, a natural analog of capsaicin, on intracellular Ca2+ level of rat thymocytes was examined using a flow-cytometric technique with appropriate fluorescent probes in order to further characterize the cytotoxicity because nonivamide can be used as an active intergradient of antifouling paints. Nonivamide at concentrations ranging from 30 μM to 300 μM significantly increased the intensity of Fluo-3 fluorescence. The potency of 100 μM nonivamide to increase the fluorescence was similar to that of 100 μM capsaicin. The increase in Fluo-3 fluorescence by 100 μM nonivamide was attenuated under an external Ca2+-free condition. Nonivamide at 100 μM also increased the intensity of Fluo-3 fluorescence in the continued presence of 100 μM capsaicin. It is suggested that nonivamide at high micromolar concentrations increases intracellular Ca2+ level via the activation of vanilloid receptors. Nonivamide concentrations (30 μM or more) that increase intracellular Ca2+ level in rat thymocytes are comparable to those in algal cells. However, it is something hard to argue the implications in environmental science because nonivamide doesn’t seem to be released into environment in such a high concentration, and because bioaccumulation of nonivamide has not been reported.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
28
Issue
3
Start Page
15
End Page
19
Sort Key
15
Published Date
2014-07
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry