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ID 109370
Title Alternative
Cyclopamineは大腸癌癌幹細胞のソニック・ヘッジホッグとその下流遺伝子発現を抑制する
Sonic Hedgehog Downstream Genes in Cancer Stem Cells
Author
Batsaikhan, Bat-Erdene The University of Tokushima
Kurita, Nobuhiro The University of Tokushima KAKEN Search Researchers
Keywords
Cancer stem-like cells
epithelial-mesenchymal transition
cancer cells
sonic hedgehog
Content Type
Thesis or Dissertation
Description
Backround: Most solid cancers including colon cancer are believed to be initiated from and maintained by cancer stem cells (CSCs), that are responsible for treatment resistance, resulting in tumor relapse. The aim of this study was to clarify the possible role of the Sonic Hedgehog (Shh) signaling pathway in the regulation of cancer stem cells. Materials and Methods: The HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Gene expressions were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) from cells treated with and without cyclopamine. Results: HCT-116 sphere-derived cells grown in serum-free, non-adherent culture, showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and down-regulated by cyclopamine treatment in a dose-dependent manner. Conclusion: Overall, these findings show that cyclopamine treatment could down-regulate the expression of stemness markers, shh downstream genes and EMT markers on HCT-116 spheres.
Journal Title
Anticancer Research
ISSN
02507005
17917530
NCID
AA10625860
AA12440673
Publisher
The International Institute of Anticancer Research
Volume
34
Issue
11
Start Page
6339
End Page
6344
Published Date
2014-11-03
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505251001.pdf
論文本文 : k2796_fulltext.pdf
本論文は,著者BAT-ERDENE BATSAIKHANの学位論文として提出され, 学位審査・授与の対象となっている。
著者の申請により要約(2015-05-25公開)に替えて論文全文を公開(2022-12-14)
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2796号
Diploma Number
甲医第1238号
Granted Date
2015-03-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Liberal Arts and Sciences
Medical Sciences