number of access : ?
number of downloads : ?
ID 109398
Title Alternative
C2C12筋管細胞においてフラボンはLPS誘導性atrogin-1/MAFbxの発現を抑制する
Flavones Prevent LPS-Induced Muscle Atrophy
Author
Shiota, Chieko Tokushima University
Ohno, Ayako Tokushima University
Keywords
flavones
LPS
muscle atrophy
JNK
Content Type
Thesis or Dissertation
Description
Muscle atrophy is a complex process that occurs as a consequence of various stress events. Muscle atrophy-associated genes (atrogenes) such as atrogin-1/MAFbx and MuRF-1 are induced early in the atrophy process, and the increase in their expression precedes the loss of muscle weight. Although antioxidative nutrients suppress atrogene expression in skeletal muscle cells, the inhibitory effects of flavonoids on inflammation-induced atrogin-1/MAFbx expression have not been clarified. Here, we investigated the inhibitory effects of flavonoids on lipopolysaccharide (LPS)-induced atrogin-1/MAFbx expression. We examined whether nine flavonoids belonging to six flavonoid categories inhibited atrogin-1/MAFbx expression in mouse C2C12 myotubes. Two major flavones, apigenin and luteolin, displayed potent inhibitory effects on atrogin-1/MAFbx expression. The pretreatment with apigenin and luteolin significantly prevented the decrease in C2C12 myotube diameter caused by LPS stimulation. Importantly, the pretreatment of LPS-stimulated myoblasts with these flavones significantly inhibited LPS-induced JNK phosphorylation in C2C12 myotubes, resulting in the significant suppression of atrogin-1/MAFbx promoter activity. These results suggest that apigenin and luteolin, prevent LPS-mediated atrogin-1/MAFbx expression through the inhibition of the JNK signaling pathway in C2C12 myotubes. Thus, these flavones, apigenin and luteolin, may be promising agents to prevent LPS-induced muscle atrophy.
Journal Title
Journal of Nutritional Science and Vitaminology
ISSN
18817742
03014800
NCID
AA00703822
Publisher
The Vitamin Society of Japan|Japan Society of Nutrition and Food Science|Center for Academic Publications Japan
Volume
61
Issue
2
Start Page
188
End Page
194
Published Date
2015-06-08
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201505281004.pdf
審査要旨 : LID201505281005.pdf
論文本文 : LID201506091001.pdf
本論文は, 著者Chieko SHIOTAの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© The Vitamin Society of Japan, Japan Society of Nutrition and Food Science
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2813号
Diploma Number
甲口第395号
Granted Date
2015-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Medical Sciences
Oral Sciences
University Hospital