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ID 109399
Title Alternative
ヒト歯肉線維芽細胞における酸化ストレスに対するレスベラトロール,ケルセチン,及びN-アセチルシステインの生物学的影響
Author
Keywords
human gingival fibroblasts
periodontitis
resveratrol
quercetin
N-acetylcysteine
Content Type
Thesis or Dissertation
Description
In periodontitis, production of reactive oxygen species (ROS) by neutrophils induces oxidative stress and deteriorates surrounding tissues. Antioxidants reduce damage caused by ROS and are used to treat diseases involving oxidative stress. This study summarizes the different effects of resveratrol, quercetin, and N-acetylcysteine (NAC) on human gingival fibroblasts (HGFs) under oxidative stress induced by hydrogen peroxide. Real-time cytotoxicity analyses reveals that resveratrol and quercetin enhanced cell proliferation even under oxidative stress. Of the antioxidants tested, resveratrol is the most effective at inhibiting ROS production. HGFs incubated with resveratrol and quercetin up-regulate the transcription of type I collagen gene after 3 h, but only resveratrol sustained this up-regulation for 24 h. A measurement of the oxygen consumption rate (OCR, mitochondrial respiration) shows that resveratrol generates the highest maximal respiratory capacity, followed by quercetin and NAC. Simultaneous measurement of OCR and the extracellular acidification rate (non-mitochondrial respiration) reveals that resveratrol and quercetin induce an increase in mitochondrial respiration when compared with untreated cells. NAC treatment consumes less oxygen and enhances more non-mitochondrial respiration. In conclusion, resveratrol is the most effective antioxidant in terms of real-time cytotoxicity analysis, reduction of ROS production, and enhancement of type I collagen synthesis and mitochondrial respiration in HGFs.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
18805086
09120009
NCID
AA10710201
AA12091064
Publisher
SOCIETY FOR FREE RADICAL RESEARCH JAPAN
Volume
56
Issue
3
Start Page
220
End Page
227
Published Date
2015-05-01
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201505291001.pdf
審査要旨 : LID201505291002.pdf
論文本文 : LID201507171007.pdf
本論文は, 著者Rita Cristina Orihuela-Camposの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
Copyright © 2015 日本酸化ストレス学会(Society for Free Radical Research Japan)
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2810号
Diploma Number
甲口第392号
Granted Date
2015-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
Bioscience and Bioindustry
Medical Sciences