ID 109577
Author
Oka, Makiko Department of Nutrition and Metabolism, the University of Tokushima Graduate School
Niwa, Yasuharu The Radiation Effects Research Foundation
Mawatari, Kazuaki Department of Nutrition and Metabolism, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hiasa, Yoshikazu The Tokushima Red Cross Hospital
Nakaya, Yutaka Department of Nutrition and Metabolism, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
endothelin-1(1-31)
sandwich-ELISA
myocardial infarction
PTCA
Content Type
Journal Article
Description
Human chymase converts big endothelin (ET)-1 to 31-amino acid length ET-1 {ET-1(1-31)} that also possesses a potent vasoconstrictive action. In addition, ET-1(1-31) is an intermediate peptide, which is then readily transformed to mature ET-1 by the neutral endopeptidase 24-11. To investigate the relevance of pathophysiology of ET-1(1-31) in vivo, we have developed specific sandwich-type, enzyme-linked immunosorbent assay to measure the plasma concentration of ET-1(1-31) in healthy volunteers and patients with myocardial infarction. The plasma concentrations of ET-1(1-31) in healthy volunteers were 24.8±5.2 pg/ml (n=11). ET-1(1-31) concentration in plasma was elevated in patients with acute myocardial infarction, and its elevation was several times higher and lasted longer than that of ET-1. In addition, tissue concentration of ET-1(1-31) in the myocardium from a patient with acute myocardial infarction was extremely high (12729.8±2617.7 pg/mg protein). These results suggest that ET-1(1-31) may play some pathological roles in the remodeling, especially in sites where inflammatory cells produced a large amount of proteases, such as myocardial infarction.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
61
Issue
3-4
Start Page
298
End Page
305
Sort Key
298
Published Date
2014-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences