ID 109625
Title Transcription
トリプル ネガティブ ニュウガン ニオケル プロテアソーム カンレン インシ PAG1 ニヨル シンキ ゾウショク キコウ ノ カイメイ
Title Alternative
Involvement of proteasome-associated gene1 in proliferation of triple negative breast cancer
Author
Komatsu, Masato Division of Genome Medicine, Institute for Genome Research, University of Tokushima
Yoshimaru, Tetsuro Division of Genome Medicine, Institute for Genome Research, University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Matsuo, Taisuke Division of Genome Medicine, Institute for Genome Research, University of Tokushima
Kiyotani, Kazuma Division of Genome Medicine, Institute for Genome Research, University of Tokushima
Yanai, Ayako Division of Genome Medicine, Institute for Genome Research, University of Tokushima|Department of Surgery, Division of Breast and Endocrine Surgery, Hyogo College of Medicine
Saito, Ayumu Laboratory of DNA information analysis, Human Genome Center, Institute of Medical Science, University of Tokyo
Yamaguchi, Rui Laboratory of DNA information analysis, Human Genome Center, Institute of Medical Science, University of Tokyo
Ono, Masaya Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute
Nakamura, Yusuke Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo
Miyoshi, Yasuo Department of Surgery, Division of Breast and Endocrine Surgery, Hyogo College of Medicine
Miyano, Satoru Laboratory of DNA information analysis, Human Genome Center, Institute of Medical Science, University of Tokyo
Sasa, Mitsunori Tokushima Breast Care Clinic
Katagiri, Toyomasa Division of Genome Medicine, Institute for Genome Research, University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Triple Negative Breast Cancer
gene-expression profiling
proteasome-associated-genes
Content Type
Journal Article
Description
Triple negative breast cancer (TNBC) is considered to be one of the most aggressive subtypes of all breast cancers. To identify novel potential therapeutic targets and clarify pathophysiological features for TNBC, we conducted Meta-gene profiling analysis based on gene-expression profiling of TNBC cases purified by lasermicrobeam microdissection, and found that proteasome-associated genes (PAGs) were commonly upregulated in various pathways including cell cycle regulation in TNBC. Depletion of PAGs with RNAi caused the upregulation of p27 and p21 proteins in MDA-MB-231 and HCC1937 cells, respectively, resulting in growth inhibition. Interestingly, immunocytochmical staining revealed that PAG1 was observed in the nucleoli and/or cytoplasm (n-PAG1 and c-PAG1) in TNBC cell line and clinical specimens. Immunohistochemical staining of 100 TNBCs showed that high level of n-PAG1 was significantly associated with poor disease free and overall survival of TNBC patients. These results indicate that n-PAG1 plays a critical role in nucleus during cell cycle progression and might be a novel prognostic indicator or an attractive molecular target of TNBC.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
69
Issue
3-4
Start Page
129
End Page
132
Sort Key
129
Published Date
2013-08-25
FullText File
language
jpn
TextVersion
Publisher
departments
Institute of Advanced Medical Sciences