ID 110153
Author
Nishitani, Chikako NARO Institute of Fruit Tree and Tea Science
Hirai, Narumi NARO Institute of Fruit Tree and Tea Science
Komori, Sadao Faculty of Agriculture, Iwate University
Wada, Masato NARO Institute of Fruit Tree and Tea Science
Okada, Kazuma NARO Institute of Fruit Tree and Tea Science
Yamamoto, Toshiya NARO Institute of Fruit Tree and Tea Science
Content Type
Journal Article
Description
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA, and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome.
Journal Title
Scientific Reports
ISSN
20452322
Volume
6
Start Page
31481
Sort Key
31481
Published Date
2016-08-17
Remark
Copyright ©2016 Authors.This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry