ID 110154
Author
Tanihara, Fuminori Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University|Diabetes Therapeutics and Research Center, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takemoto, Tatsuya Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kitagawa, Eri Research and Development Center, NH Foods Ltd
Rao, Shengbin Research and Development Center, NH Foods Ltd
Kim Do, Lanh Thi Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University
Onishi, Akira Laboratory of Animal Reproduction, Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University|Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
Yamashita, Yukiko Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University
Kosugi, Chisato Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University
Suzuki, Hitomi Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University
Sembon, Shoichiro Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
Suzuki, Shunichi Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
Nakai, Michiko Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
Hashimoto, Masakazu Laboratory for Embryogenesis, Graduate School of Frontier Biosciences, Osaka University
Yasue, Akihiro Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Matsuhisa, Munehide Diabetes Therapeutics and Research Center, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Noji, Sumihare Department of Life System, Institute of Technology and Science, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Fujimura, Tatsuya Research and Development Center, NH Foods Ltd
Fuchimoto, Dai-ichiro Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
Otoi, Takeshige Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Content Type
Journal Article
Description
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing in pigs that involves the introduction of Cas9 protein and single-guide RNA into in vitro fertilized zygotes by electroporation. The use of gene editing by electroporation of Cas9 protein (GEEP) resulted in highly efficient targeted gene disruption and was validated by the efficient production of Myostatin mutant pigs. Because GEEP does not require the complex methods associated with micromanipulation for somatic reprogramming, it has the potential for facilitating the genetic modification of pigs.
Journal Title
Science Advances
ISSN
23752548
Volume
2
Issue
9
Start Page
e1600803
Sort Key
1600803
Published Date
2016-9-14
Remark
2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry
Institute of Advanced Medical Sciences
University Hospital