ID 110164
Author
Ueta, Risa Graduate School of Advanced Technology and Science, Tokushima University
Abe, Chihiro Graduate School of Advanced Technology and Science, Tokushima University
Watanabe, Takahiro Center for Collaboration among Agriculture, Industry, and Commerce, Tokushima University
Sugano, Shigeo S Center for Collaboration among Agriculture, Industry, and Commerce, Tokushima University
Ishihara, Ryosuke Graduate School of Advanced Technology and Science, Tokushima University
Ezura, Hiroshi Graduate School of Life and Environmental Sciences, University of Tsukuba
Content Type
Journal Article
Description
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9—a key gene controlling parthenocarpy— with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit—a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.
Journal Title
Scientific Reports
ISSN
20452322
Volume
7
Start Page
507
Sort Key
507
Published Date
2017-03-30
Remark
© The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry