ID 110327
Title Transcription
メンタル ヘルス オ ササエル アラタナ ストレス バイオ マーカー
Title Alternative
A novel biomental tool for assessing psychological stress response
Author
Kuwano, Yuki Departments of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Katsuura, Sakurako Departments of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Masuda, Kiyoshi Departments of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Tanahashi, Toshihito Departments of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School
Rokutan, Kazuhito Departments of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
microarray
gene expression
psychological stress
alternative splicing
Content Type
Journal Article
Description
Stress plays an important role in both mental and physical problems. Stressful life events initiate a coordinated physiological process that is produced by interactions between the hypothalamuspituitary- adrenal axis, sympathetic nervous system, and immune system. The response to psychological stress varies considerably and depends on a wide range of environmental and genetic factors. Establishment of a new biomental tool for objectively assessing stress response is required. We focus on high-throughput analysis of gene expression using microarray system to study the complex stress responses. Alternative splicing(AS)regulates the gene expression program in response to surrounding environment. However, acute psychological stress-initiated AS events have not been documented yet. Academic examinations are one of the brief naturalistic stressors and have been shown to change gene expression in peripheral leukocytes, which is postulated to be involved in the psychological response. Using the GeneChip human exon1.0ST array, AS events of 27 genes with splicing indices >1.0could be detected immediately after the examination among healthy university students. Real-time reverse transcription PCR validated the stress-initiated skipping of exon 63 of SMG -1 that encodes a phosphatidylinositol 3-kinase-related protein kinase crucial for activations of p 53-dependent pathways and mRNA decay system. These results indicate that AS mediated regulation of gene expression in response to brief naturalistic stressors in peripheral leukocytes, and suggest the SMG -1 splice variant as a potential biomarker for acute psychological stress.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
66
Issue
5-6
Start Page
119
End Page
122
Sort Key
119
Published Date
2010-12-20
Remark
p.122 著者英語表記誤記あり(誤)Toshihiko Tanahashi →(正)Toshihito Tanahashi
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences