ID 110419
Author
Niwa, Eiko Faculty of Integrated Arts and Sciences, Tokushima University
Mitani, Tsuyoshi Faculty of Integrated Arts and Sciences, Tokushima University
Saitoh, Shohei Faculty of Integrated Arts and Sciences, Tokushima University
Ishida, Shiro Faculty of Pharmaceutical Sciences, Tokushima Bunri University
Oyama, Yasuo Faculty of Bioscience and Bio-industry, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
arachidonic acid
cytotoxicity
lymphocyte
oxidative stress
zinc
Content Type
Journal Article
Description
Previous studies on the cytotoxicity of arachidonic acid (ARA) elucidated the involvement of oxidative stress and Ca2+. In the present study, the Zn2+-related cytotoxicity of ARA was studied by a flow cytometric technique with appropriate fluorescent probes in rat thymocytes. Addition of 10 μM ZnCl2 enhanced the increase in cell lethality induced by 10 μM ARA. The removal of Zn2+ by Zn2+ chelators attenuated the ARA-induced increase in cell lethality. Thus, Zn2+ is suggested to be involved in ARA cytotoxicity. ARA at 3–10 μM elevated intracellular Zn2+ level. The Zn2+ chelators attenuated the ARA-induced increase in intracellular Zn2+ level while ARA significantly increased intracellular Zn2+ level in the presence of 3 μM ZnCl2, suggesting the involvement of external Zn2+. Zn2+ reportedly exerts cytotoxic action under oxidative stress induced by hydrogen peroxide, via an excessive increase in intracellular Zn2+ levels. Since ARA induces oxidative stress, the simultaneous administration of zinc and ARA may be harmful.
Journal Title
Food and Chemical Toxicology
ISSN
02786915
NCID
AA10627174
AA11526376
Publisher
Elsevier
Volume
96
Start Page
177
End Page
182
Sort Key
177
Published Date
2016-08-08
Remark
Copyright © 2016 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry