ID 110455
Title Transcription
チュウスウ ゼンテイケイ ニオケル ヘイコウ ダイショウ ノ ブンシ キコウ
Title Alternative
Molecular mechanisms of vestibular compensation in the central vestibular system
Author
Takeda, Noriaki Department of Otolaryngology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
vestibular compensation
cerebellum
protein phosphatase
glutamate receptor
neural plasticity
Content Type
Journal Article
Description
The differential display method was applied to identify genes expression of which is upordown-regulated in the flocculus after unilateral labyrinthectomy (UL) in rats. TotalRNAs from sham operated flocculus and labyrinthectomized flocculus were isolated andamplified by PCR using arbitrary primer sets. PCR products the amounts of which weresignificantly higher or lower in samples from operated animals than those from controls,were cut out of the gel and sequenced. One of the higher expressed fragments showed100% nucleotide sequence identify to protein phosphatase 2A (PP2A) beta catalytic subunitmRNA. In animals with continuous floccular infusion of okadaic acid, a potent inhibitor ofPP 2 A, UL-induced spontaneous nystagmus (SN) lasted significantly longer. One of the lowerexpressed fragments showed 100% nucleotide sequence identify to glutamate receptor (GluR)delta-2 subunit mRNA. UL-induced SN in GluR delta-2 mutant mice was exacerbated at theinitial stage. All these findings suggest that up-regulation of PP2A beta and down regulateGluR delta-2 in flocculus after UL is involved in lesion-induced vestibular plasticity.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
56
Issue
4
Start Page
132
End Page
135
Sort Key
132
Published Date
2000-08-25
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences