ID 110492
Title Transcription
DHPLC Denaturing High Performance Liquid Chromatography オ モチイタ ダンジョ シキベツ
Title Alternative
A Novel Method for Sex Identification by Analysis of a heteroduplex using Denaturing High Performance Liquid Chromatography (DHPLC)
Author
Shinka, Toshikatsu Department of Public Health, The University of Tokushima School of Medicine
Naroda, Takushi Department of Public Health, The University of Tokushima School of Medicine|Department of Urology, The University of Tokushima School of Medicine
Tamura, Takamichi Department of Public Health, The University of Tokushima School of Medicine
Unemi, Yukiko Department of Public Health, The University of Tokushima School of Medicine
Tsuji, Keiko Department of Public Health, The University of Tokushima School of Medicine
Sasahara, Kenji Department of Public Health, The University of Tokushima School of Medicine
Nakahori, Yutaka Department of Public Health, The University of Tokushima School of Medicine
Keywords
Sex idetification
amelogenin
heteloduplex
DHPLC
Content Type
Journal Article
Description
A novel method for sex identification using a denaturing high performance liquid chromatography (DHPLC) system is described. Among many methods for identifying sex, the most popular and credible system has been the polymerase chain reaction (PCR) method using the nucleotide primer sets of the amelogenin gene, which is shared on both the X and Y chromosomes. In this conventional method, the judgment depends on the detection of the size difference between the PCR products derived from the X and Y chromosomes. In this study, we adopted DHPLC to detect the difference by checking heteroduplex formation between the products, which enabled us to shorten the PCR products down to 45 bp and the separation time within 8 minutes. This new system may have a wide application in many different fields such as forensic medicine, prenatal diagnosis, inbreeding of animals and anthropology.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
57
Issue
3
Start Page
79
End Page
83
Sort Key
79
Published Date
2001-06-25
FullText File
language
jpn
TextVersion
Publisher