ID 110583
Title Transcription
ダイチョウ ジョウヒ サイボウ ニ ハツゲン スル NADPH oxidase1 Nox1 ノ ブンシ トクセイ
Title Alternative
Molecular characterization of NADPH oxidase1 expressed on large intestinal epithelial cells
Author
Kuwano, Yuki Depertment of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kawahara, Tsukasa Depertment of Nutritional Physiology, The University of Tokushima School of Medicine
Kodama, Nanae Depertment of Nutritional Physiology, The University of Tokushima School of Medicine
Nakai, Saori Depertment of Nutritional Physiology, The University of Tokushima School of Medicine
Kishi, Kyouichi Depertment of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Rokutan, Kazuhito Depertment of Nutritional Physiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Nox1
large intestinal epithelial cells
Toll-like receptor 5
flagellin
Content Type
Others
Description
NADPH oxidase 1 (Nox1) is an isozyme of gp91-phox predominantly expressed in the human colon. In this study, we have established primary cultures of guinea pig large intestinal epithelial cells (LIEC). A great majority of the cultured cells (>90%) was surface mucous cells containing periodic acid-Schiff reaction-positive granules. Vimentin-positive fibroblasts were <1%, and macrophages were not contaminated. LIEC spontaneously produced superoxide anion (O2 -) at about 160 nmol/mg protein/h. O2 --dependent formation of blue formazan particles from nitroblue tetrazolium was observed only on surface of mucous-producing cells, and these cells expressed Nox1 protein at plasma membrane and in the cytoplasm. They expressed p67-phox, p22-phox, and rac1, but not gp91-phox, p47-phox, p40-phox, and rac 2. Immunohistochemistry showed that Nox1, p 67-phox, and p 22-phox were predominantly expressed in surface mucous cells of human and guinea pig colonic mucosa. Human colon cancer cell lines (Caco2, T84, and HT29 cells) expressed Nox1, p22-phox, and rac1, but not the other NADPH components. These cells secreted O2 - at <5 nmol/mg protein/h. Caco2 cells possessed Toll-like receptor 5, and flagellin (FliC) from Salmonella enteritidis phosphorylated transforming growth factor-β-activated kinase 1 (TAK1) and TAK1-binding protein 1, and significantly up-regulated O2 - production. These results suggest that Nox1 expressed in colonic epithelial cells may regulate interactions between pathogenic bacteria and epithelial cells for host defense.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
59
Issue
1-2
Start Page
45
End Page
46
Sort Key
45
Published Date
2003-04-25
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences