ID 110631
Author
Intiyot, Yaowarate Department of Bacteriology, The University of Tokushima School of Medicine|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Kinouchi, Takemi Department of Bacteriology, The University of Tokushima School of Medicine
Kataoka, Keiko Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arimochi, Hideki Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kuwahara, Tomomi Department of Bacteriology, The University of Tokushima School of Medicine
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Ohnishi, Yoshinari Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Keywords
Murdannia loriformis
antimutagenicity
azoxymethane-induced aberrant crypt foci
O6-methylguanine
Content Type
Journal Article
Description
An 80% ethanol extract of Murdannia loriformis, a Thai medicinal plant, was examined for antimutagenic activity and cancer chemopreventive activity. In the Salmonella mutation assay, the extract showed antimutagenicity against 2-amino-3-methylimidazo [4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline, 2-amino-3,8-dimethylimidazo [4,5-f]quinoxaline, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, 2-amino-1,4-dimethyl- 5H-pyrido[4,3-b]indole, 3-amino-1-methyl-5H-pyrido[4,3-b]indole, 2-amino-6-methyldipyrido [1,2-a:3’,2’-d] imidazole, 2-aminodipyrido[1,2-a:3’,2’-d]imidazole, 2-aminoanthracene, 2-(2- furyl)-3-(5-nitro-2-furyl) acrylamide,N-methyl-N’-nitro-N-nitrosoguanidineandmethylazoxymethanol acetate and reduced their mutagenicities to 31.4~67.9% at the dose of 10mg/plate. However, it did not inhibit the mutagenicities of 2-amino-9H-pyrido[2,3-b]indole, 2-amino-3-methyl-9 H-pyrido[2,3-b]indole, benzo[a]pyrene,N-ethyl-N’-nitro-N-nitrosoguanidine and 1-nitropyrene. The extract itself showed no mutagenicity. The chemopreventive activity of M. loriformis was examined using azoxymethane (AOM)-induced aberrant crypt focus (ACF) formation in the colon of F344 rats. The extract at doses of 0.1-1.0 g/kg wt significantly inhibited ACF formation in the initiation stage (21-51%), although it wasmore effective at a lower dose. In the post-initiation stage, the extract also tended to inhibit ACF formation (12 -27%) and significantly decreased the number of larger ACFs that have more than 3 aberrant crypts per focus. The extract inhibited the formation of O6-methylguanine and N7-methylguanine in the colonic mucosa and muscular layers but not or increased in the liver. These results indicate that M. loriformis extract has antimutagenic activity toward variousknownmutagens and that it inhibits AOM-induced ACF formation both in the initiation and post-initiation stages in the rat colon.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
49
Issue
1-2
Start Page
25
End Page
34
Sort Key
25
Published Date
2002
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences