ID 110675
Author
Hayashi, Yoshio Department of Pathology, Tokushima University School of Dentistry Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arakaki, Rieko Department of Pathology, Tokushima University School of Dentistry Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ishimaru, Naozumi Department of Pathology, Tokushima University School of Dentistry Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Sjögren’s syndrome
autoantigen
caspase
apoptosis
Content Type
Journal Article
Description
Primary Sjögren syndrome (SS) is an autoimmune disease characterized by diffuse lymphoid cell infiltrates in the salivary and lacrimal glands, resulting in symptoms of dry eye and dry mouth due to insufficient secretion. Previously, we have identified the120 kDa α-fodrin as an important autoantigen on the development of SS in both animal model and SS patients, but the mechanism of α-fodrin cleavage leading to tissue destruction in SS remains unclear. In murine primary SS model, tissue-infiltrating CD4+ T cells purified from the salivary glands bear a large proportion of Fas ligand (FasL), and the salivary gland duct cells constitutively possess Fas. Infiltrating CD4+ T cells identified significant51Cr release against mouse salivary gland (MSG) cells. In vitro studies demonstrated that apoptotic MSG cells result in a specific α-fodrin cleavage into 120 kDa, and preincubation with caspase-inhibitor peptides blocked α-fodrin cleavage. The treatment with caspase-inhibitors in vivo prevented the development of autoimmune lesions in the salivary and lacrimal glands. Thus, an increased activity in caspase cascade may be involved in the progression of α-fodrin proteolysis and tissue destruction on the development of SS.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
50
Issue
1-2
Start Page
32
End Page
38
Sort Key
32
Published Date
2003
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences