ID 110685
Author
Ishigami, Akiko Department of Legal Medicine, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kubo, Shin-ichi Department of Legal Medicine, The University of Tokushima School of Medicine
Gotohda, Takako Department of Legal Medicine, The University of Tokushima School of Medicine
Tokunaga, Itsuo Department of Legal Medicine, The University of Tokushima School of Medicine
Keywords
methamphetamine
kidney
myoglobin
the 70 kDa heat shock protein
immunohistochemistry
Content Type
Journal Article
Description
Forensic autopsy cases detecting methamphetamine (MA) are usually diagnosed according to its toxicological concentration. It has been reported that the lethal blood concentration of MA is 4.48 μg/ml (3.0μmol/dl). We autopsied two MA-detected cadavers, and immunohistochemical staining was performed on the skeletal muscle with an anti-myoglobin antibody, and on the kidney with an anti-the 70 kDa heat shock protein(HSP70) antibody. One case showed a high rectal temperature (40℃). The toxicological examination revealed 0.75μg/ml of MA in the blood, and 16.8 μg/ml in the urine. Myoglobin was negative and HSP70 was positive in the kidney immunohistochemically. From the toxicological and immunohistochemical findings, it was considered that the subject died of hyperthermia and acidosis caused by muscular hyperactivity. In another case, the autopsy revealed highly congested lungs, with dark-red bloody fluid and foam in the trachea and bronchus. MA (17.0 μg/ml) was detected in the blood. HSP70 was negative and myoglobin was positive immunohistochemically. It was thought that the subject died of acute MA intoxication based on the high MA concentration, although rhabdomyolysis was suspected. It is suggested that myoglobin and HSP70 immunostaining are useful to diagnose MA poisoning.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
50
Issue
1-2
Start Page
112
End Page
116
Sort Key
112
Published Date
2003
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences