ID 110716
Author
Sakai, Manabu Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine
Miyake, Hidenori Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine
Tashiro, Seiki Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Okumura, Yuushi Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima KAKEN Search Researchers
Kido, Hiroshi Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
FK 506
cyclosporine A
liver cancer cell
invasion
ICAM-1
Content Type
Journal Article
Description
Background : The prognosis of liver transplantation for liver cancer is determined by recurrence in the liver graft. In this study, the effects of immunosuppressors, FK506 and cyclosporine A (CsA) on the migration of liver cancer cells were investigated. Methods : The effects of FK506 at concentrations of 1-100 ng/mL and CsA at 1-1000ng/mL on the growth of poorly and well differentiated human hepatocellular carcinoma cell lines, HLE and HuH-7, respectively, were examined. After treatment of these cells with FK506 and CsA, the growth of these cells, their cytotoxicities and invasion assay on the Matrigel basement membrane invasion chamber were evaluated. In addition, the effects of FK506 and CsA on the changes in the production of a soluble intercellular adhesionmolecule-1 (sICAM-1) of these cells were measured. Results : FK506 and CsA at concentrations of 1-10 ng/mL inhibited the growth of both HLE and HuH-7 and those immunosuppressors at concentrations over 100 ng/mL exhibited cytotoxicity on these cells. FK506 at concentration of 1ng/mL significantly inhibited the invasion of poorly differentiated HLE, but not well differentiated HuH-7, after treatment for 2-5 days in culture (p<0.05), but FK 506 at 10 ng/mL showed less inhibitory efficient. CsA at concentrations of 1-10 ng/mL, however, did not inhibit or transiently inhibited the invasion of both cell lines. The production of ICAM-1 in HLE and HuH-7 was suppressed by FK506 at concentrations of 1-10 ng/mL after treatment for 3-5 days but the effect was not significant in the initial phase at days 1-2 and the last phase at days 5-6. Conclusions : FK506, but not CsA, at a clinical dose of 1ng/mL significantly inhibited the invasion of the poorly-differentiated HLE, but not HuH-7 and also inhibited the production of sICAM-1 in HLE.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
51
Issue
1-2
Start Page
63
End Page
69
Sort Key
63
Published Date
2004-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Institute of Advanced Medical Sciences
Medical Sciences