ID 110739
Author
Ikemoto, Tetsuya Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Tashiro, Seiki Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Yasutomo, Koji Department of Immunology and Parasitology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kishihara, Kenji Department of Immunology and Parasitology, The University of Tokushima School of Medicine
Kurita, Nobuhiro Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine KAKEN Search Researchers
Miyake, Hidenori Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine
Keywords
islet transplantation
experimental transplantation
donor-specific tolerance
CD4+CD25+ T-cells
mouse
Content Type
Journal Article
Description
Background. The allogeneic islets transplantation is an ideal therapeutic strategy for patients with diabetes mellitus. However, it has been difficult to induce immunological tolerance against islets grafts. TheCD4+CD25+ regulatory T-cells (Treg) play a role in suppressing T-cell activation. Thus, we evaluated whether Treg can regulate donor-specific T-cell tolerance that received allogeneic islets into the hepatic parenchyma (ITxHP) along with Treg. Methods. C3H/He mice were used as donors and streptozotocin-induced diabetic BALB/c mice were recipients. The protocol included three groups : Group A recipients received only 300 IE islets Group B was given 300 IE islets and whole splenocytes Group C was given 300 IE islets and Treg purified from peripheral lymph nodes. Results. For all mice in Groups A and B, the fasting blood sugar exceeded 250mg/dl and graft rejection was observed. GVHD was observed earlier in Group B than in Group A. In contrast graft survival exceeded 30 days for two mice in Group C (50%, mean POD 28.5±24.0, Plt0.05). Mixed lymphocyte reaction showed that T-cells from tolerant mice had very weak responses against spleen cells from C3H mice. Conclusions. The simultaneous ITxHP with CD4+CD25+ T-cells administration prolonged islet graft survivals and induced donor-specific hyporesposiveness.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
51
Issue
3-4
Start Page
178
End Page
185
Sort Key
178
Published Date
2004-08
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
University Hospital
Medical Sciences