ID 110764
Author
Masuda, Aki Divisions for Animal Research Resources, Institute of Health Biosciences, The University of Tokushima Graduate School
Kose, Hiroyuki Divisions for Animal Research Resources, Institute of Health Biosciences, The University of Tokushima Graduate School
Matsumoto, Kozo Divisions for Animal Research Resources, Institute of Health Biosciences, The University of Tokushima Graduate School
Keywords
QTL
congenic strain
OLETF
type 2 diabetes
Content Type
Journal Article
Description
Linkage analysis previously identified ahyperglycemic quantitative trait locus (QTL), Nidd 2/of, on rat Chromosome 14 in crosses utilizing OLETF (Otsuka Long Evans Tokushima Fatty) rat, a model for type 2 diabetes. A separate QTL study mapped an obesity QTL, Obs5, to the same chromosomal region. A congenic strain placing ca. 38cMOLETF-derivedsegments containing both Nidd2/of and Obs5 on the F344 background was shown to possess mild diabetic and obese phenotypes, suggesting the presence of mutations affecting the glucose metabolism and fat accumulation. In order to localize the loci more precisely, we generated a series of deletionsubcongenic strains in which OLETF-segments were shortened from either ends. We found that there are at least two hyperglycemic QTLs within the Nidd2/of locus. We predict that they are localized towards both ends of the Nidd2/of region. In contrast, Obs5QTL was further narrowed down to a single region of ca. 10 cM fragment.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
52
Issue
1-2
Start Page
109
End Page
113
Sort Key
109
Published Date
2005-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher