ID 110787
Author
Shindo, Tokuhisa Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Matsumoto, Yoshihito Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Wang, Qinghua Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Kawai, Nobuyuki Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Tamiya, Takashi Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Nagao, Seigo Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Keywords
embryonic stem cell
neural stem cell
transplantation
cholinergic
traumatic brain injury
Content Type
Journal Article
Description
We developed a novel protocol for generation and selective amplification of neural progenitor cells regionally specified to the rostral brain but not the spinal cord from mouse embryonic stem cells (ESCs). The neural progenitors could differentiate in vitro and in vivo into many cholinergic and a few GABAergic neurons but rarely into astrocytes. The transplanted neurospheres could survive in the hippocampus (CA3) of animals with mild traumatic brain injury (TBI). Twelve weeks after transplantation (a week after the behavioral test), we found significant cholinergic differentiation recognized as ChAT immunoreactivity in the eGFP+ transplanted cells. Moreover, the grafts contained a few GAD67+cells. However, we barely found GFAP+ astrocytes within the grafts. Furthermore, presynaptic formations of graft-derived neurons were recognized by immunohistochemistry of near the grafts aroundCA3. However, these findings were not observed in severe TBI group. So, we examined NGF, BDNF, and FGF-2 mRNA by RT-PCR in 12 mice including normal, mild TBI and severe TBI group. Increases in the neurotrophic factors’ mRNA were evident in the hippocampus on the ipsilateral side in the mild TBI group. Statistical analysis revealed significant differences between the mild and severe TBI groups. The data also revealed significant differences between the mild TBI and normal groups. The transplanted neurospheres could survive in the mild TBI animals, but not in the severe TBI group.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
42
End Page
51
Sort Key
42
Published Date
2006-02
FullText File
language
eng
TextVersion
Publisher