ID 110789
Author
Wang, Qinghua Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Matsumoto, Yoshihito Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Shindo, Tokuhisa Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Miyake, Keisuke Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Shindo, Atsushi Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Kawanishi, Masahiko Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Kawai, Nobuyuki Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Tamiya, Takashi Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Nagao, Seigo Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Keywords
neurosphere
embryonic stem cell
nucleus basalis of Meynert
dementia
mouse
Content Type
Journal Article
Description
Objective. The goal of this study was to elucidate the effect of neurospheres (NS) on dementia in the mouse model of nucleus basalis of Meynert (NBM) lesion. Methods. Mouse embryonic stem cell (ES) derived neurospheres were transplanted into the frontal association cortex and barrel field of S1 cortex of C57BL/6mice 4weeks after including a lesion of NBM by ibotenic acid, while other healthy mice that received ES cells served as control. Behavioral tests by 8-armradial maze were conducted 8 weeks after transplantation, and double staining of choline acetyltransferase (ChAT), serotonin, amyloid-βprotein (AP) and green fluorescent protein(GFP)12 weeks after transplantation.We found that the neurospheres transplanted into the mouse cortex survived and produced many ChAT-positive neurons and a few serotoninpositive neurons in and around the grafts. The working memory error decreased significantly in the mice grafted with neurospheres. In contrast, the ES cells developed into teratomas in all of the control mice and expressed no neurons, and the working memory deteriorated remarkably. Conclusions. Transplantation of neurospheres, but not ES cells, into the prefrontal and parietal cortices, dramatically alleviated the cholinergic deficits and recent memory disruption in the NBM lesioned mice.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
61
End Page
69
Sort Key
61
Published Date
2006-02
FullText File
language
eng
TextVersion
Publisher