ID 110790
Author
Alizadeh, Mohammad Department of Preventive Environment and Nutrition, Institute of Health Biosciences. The University of Tokushima Graduate School
Ota, Fusao Department of Preventive Environment and Nutrition, Institute of Health Biosciences. The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hosoi, Kazuo Department of Molecular Oral Physiology, Institute of Health Biosciences. The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kato, Makoto Department of International Public Health Nutrition, Institute of Health Biosciences. The University of Tokushima Graduate School
Sakai, Tohru Department of International Public Health Nutrition, Institute of Health Biosciences. The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Satter, Mohammed A. Institute of Food Science and Technology, Room No.1, BCSIR
Keywords
BPA
food allergy
OVA
IgE
IgG2a
cytokines
Content Type
Journal Article
Description
The Objective of this study was to elucidate if Bisphenol A (BPA) administration modulates T helper (Th) cell component of immune responses in a mouse challenged with ovalbumin (OVA), a major food antigen. BALB/c mice, (6 weeks old, female) were orally given either OVA (OVA-fed) or water (Water-fed), immunized intraperitoneally with OVA and injected with either BPA in corn oil or the vehicle alone. After subsequent 2nd immunization, serum titers of total IgE, OVA-specific IgE, IgG, IgG1 IgG2a and ability of their splenocytes for production of interferon (IFN)-γ, interleukin(IL) -4andIL-12 were examined by ELISA. Lymphocyte proliferation assay against concanavalin A (ConA) or OVA was also performed for 3H-Thymidine incorporation. In Water-fed groups, treatment with BPA resulted in lower titers of total IgE(P<0.01) and higher levels IgG2a (P<0.05) followed by a higher IFN-γ (P<0.05) and IL-12 (P<0.05) with an intact IL-4. When OVA-fed groups were examined, the compound did not change production of total and OVA-specific IgE and -IgG2a but resulted in lower production of IFN-γ (P<0.05). Also, BPA resulted in impaired lymphocyte proliferation to Con A in Water-fed groups (P<0.05) but not in tolerated animals. The findings indicate that BPA results in augmentation of Th1 immune responses but no significant effect on an established tolerance to OVA.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
70
End Page
80
Sort Key
70
Published Date
2006-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences
Oral Sciences