ID 110799
Author
Michishige, Fumiko Department of Fundamental Nursing, School of Health Sciences, The University of Tokushima
Kanno, Kyoko Department of Fundamental Nursing, School of Health Sciences, The University of Tokushima
Yoshinaga, Sumiko Department of Fundamental Nursing, School of Health Sciences, The University of Tokushima
Hinode, Daisuke Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takehisa, Yozo Hakuai Kinen Hospital
Yasuoka, Susumu Hakuai Kinen Hospital
Keywords
saliva
S-IgA
kallikrein
trypsin-like enzyme
saliva collection
Content Type
Journal Article
Description
In order to clarify how we collect saliva for analyzing salivary protein in aged subjects who cannot eat well, we compared the effects of suction, spitting and the swab saliva collection method on the yield of protein components in saliva samples from normal volunteers. The saliva collected by suction, spitting and the swab method were designated as, Saliva I, II and III, respectively. The saliva volume collected by Saliva I was about 2-fold greater than that by of Saliva II and III. This is mainly due to the fact that saliva secretion was stimulated by the suction itself. The content of total protein, S-IgA, trypsin-like activity and human airway trypsin-like protease(HAT)were almost the same in Saliva I and II, and significantly lower in Saliva III than in Saliva I and II. Kallikrein activity was almost the same in Saliva I, II and III. The concentration of eachtotal protein, S-IgA, kallikrein activity, trypsin activity and HAT in Saliva I were significantly positively correlated with that in Saliva II. These results indicate that we can obtain information of change of salivary protein by analyzing saliva collected by suction method, although this method caused the stimulation of saliva to some extent.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
140
End Page
146
Sort Key
140
Published Date
2006-02
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences