ID 110800
Author
Umeno, Mayumi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Major in Laboratory Science, School of Health Sciences, Faculty of Medicine, The University of Tokushima|Core Research for Evolutional Science and Technology Corporation
Shinka, Toshikatsu Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation
Sato, Youichi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yang, Xin-Jun Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation
Baba, Yoshinobu Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima|Department of Applied Chemistry, Graduate School of Engineering, The University of Nagoya
Iwamoto, Teruaki Department of Urology, St.Marianna Medical University School of Medicine|Core Research for Evolutional Science and Technology Corporation
Nakahori, Yutaka Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation
Keywords
Y chromosome
AZF
multiplex PCR
microchip electrophoresis
azoospermia
Content Type
Journal Article
Description
Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
147
End Page
152
Sort Key
147
Published Date
2006-02
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences