ID 110818
Author
Kogame, Masahiro Institute for Enzyme Research, The University of Tokushima|Department of Radiology, Institute of Health Biosciences, The University of Tokushima Graduate School
Matsuo, Shinji Institute for Enzyme Research, The University of Tokushima KAKEN Search Researchers
Nakatani, Masashi Institute for Enzyme Research, The University of Tokushima
Kurisaki, Akira Institute for Enzyme Research, The University of Tokushima
Nishitani, Hiromu Department of Radiology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Tsuchida, Kunihiro Institute for Enzyme Research, The University of Tokushima
Sugino, Hiromu Institute for Enzyme Research, The University of Tokushima
Keywords
ALK7
adipocyte differentiation
activin
adipogenesis
TGF-beta
Content Type
Journal Article
Description
Transforming growth factor-β (TGF-β) family members regulate a variety of cellular functions and play important roles in cell differentiation. Activin receptor-like kinase 7 (ALK7), a receptor for TGF-β family members, was initially cloned from rats as an orphan receptor and has been recently shown to be a type I receptor for nodal, activin B and activin AB. ALK7 is expressed not only in neurons, but also in insulin-producing islet β cells and white and brown adipose tissues however, the specific functions of ALK7 in these tissues are not known. In order to test whether ALK7 is involved in adipocyte differentiation, we analyzed its expression during adipocyte differentiation. ALK7 expression was detected in the late phase of adipocyte differentiation by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence staining in 3T3-L1 cells. We also detected the expression of ALK7 by RT-PCR in stromal vascular fraction (SVF) cells. These results indicated that ALK7 is a novel marker specifically expressed during the late phase of adipocyte differentiation. Furthermore, our results suggest the possible involvement of nodal or activin B in adipocyte differentiation.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
3-4
Start Page
238
End Page
245
Sort Key
238
Published Date
2006-08
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences