ID 110819
Author
Sannomiya, Katsutaka Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Honda, Hirohito Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Kubo, Kenichirou Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Ii, Kunio Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Yuan, Ying Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Aoyagi, Eriko Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Muguruma, Naoki Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Shimizu, Ichiro Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School
Ito, Susumu Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Keywords
guanase
nedasin
rat
human
Content Type
Journal Article
Description
Human guanase is known as a specific enzyme in the liver, kidney, and brain. However, its functional significance remains poorly understood. In addition, interestingly, a different organ distribution between humans and rats was suggested. Here, we performed immunohistochemical staining with anti-human nedasin (neuronal and endocrine discs large/SAP102 associated protein), whose sequence was identical to that of guanase, antibody and histochemical staining for guanase in normal tissues of rat and human liver, kidney, and small intestine, and compared the results. Guanase activity was observed uniformly in the rat hepatocytes, biliary epithelium and vascular endothelium cells, while it was localized to the hepatocytes and biliary epithelium in the human liver. When the histochemical staining for guanase and the immunohistochemical staining for nedasin were compared, the stained regions were different in the rat liver but were almost consistent in all human tissues. Totally consistent staining results were also obtained between rats and humans in the other organization except the liver. Based upon the research reports to date, the experiments on guanase and nedasin in rat organs performed in this study are considered to have important implications in the investigation of their physiological significance.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
3-4
Start Page
246
End Page
254
Sort Key
246
Published Date
2006-08
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences