ID 110822
Author
Piroozmand, Ahmad Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School
Khamsri, Boonruang Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School
Fujita, Mikako Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School
Adachi, Akio Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Uchiyama, Tsuneo Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Keywords
HIV-2
accessory proteins
Vpx
Vpr
Content Type
Journal Article
Description
We have previously shown that human immunodeficiency virus type 2 (HIV-2) without functional vpx and vpr genes is severely defective for viral growth in lymphocytic cells, and suggested that the virions produced in the absence of Vpx and Vpr are critically damaged. To examine the nature of replication-defect for the vpx/vpr double mutant, we quantitatively and morphologically studied the virions produced in cells transfected or infected with wild type clone, single (vpx and vpr mutants) or the double mutant. While no significant difference in virion production was found for various virus clones in transfected cells, a major growth retardation in infected cells was readily observed for the vpx and vpx/vpr mutants. In particular, no viral growth was detected for the double mutant. By contrast to the very distinct growth characteristics of the three mutant clones, no appreciable difference in virion morphology was noted. These results indicated that Vpx and Vpr of HIV-2 may cooperatively contribute to virion infectivity without affecting virion morphogenesis.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
3-4
Start Page
271
End Page
276
Sort Key
271
Published Date
2006-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences