ID 110862
Author
Miyoshi, Keiko Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nagata, Hideya Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Horiguchi, Taigo Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Abe, Kaori Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Wahyudi, Ivan Arie Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School
Baba, Yoshinobu Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Harada, Hidemitsu Department of Oral Anatomy II, Iwate Medical University School of Dentistry
Noma, Takafumi Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
dental epithelium
BMP2
microarray
gene profiling
Content Type
Journal Article
Description
Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (BMP2) is known as one of the inducers for tooth development. To analyze the molecular mechanisms of BMP2 on ameloblast differentiation (amelogenesis), we performed microarray analyses using rat dental epithelial cell line, HAT-7. After confirming that BMP2 could activate the canonical BMP-Smads signaling in HAT-7 cells, we analyzed the effects of BMP2 on 14,815 gene expressions and profiled them. Seventy-three genes were up-regulated and 28 genes were down-regulated by BMP2 treatment for 24 hours in HAT-7 cells. Functional classification revealed that 18% of up-regulated genes were ECM/adhesion molecules present in the enamel organ. Furthermore, we examined the expression of several differentiation markers in dental epithelial four cell-lineages including inner enamel epithelium (ameloblasts), stratum intermedium, stratum reticulum, and outer enamel epithelium. The results indicated that BMP2 might induce at least two different cell-lineage markers including a BMP antagonist expressed in HAT-7 cells, suggesting that BMP2 could accelerate amelogenesis via BMP signaling.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
55
Issue
3-4
Start Page
216
End Page
226
Sort Key
216
Published Date
2008-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences
University Hospital